Structure and organization of rRNA operons In the region of the replication origin of theBacillus subtilischromosome | Zendy

Naotake Ogasawara | Zendy; Shigeki Moriya | Zendy; Hiroshi Yoshikawa | Zendy

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Structure and organization of rRNA operons In the region of the replication origin of theBacillus subtilischromosome

Author(s) -

Naotake Ogasawara,

Shigeki Moriya,

Hiroshi Yoshikawa

Publication year - 1983

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/11.18.6301

Subject(s) - biology , 23s ribosomal rna , ribosomal rna , genetics , operon , transfer rna , gene , 5s ribosomal rna , internal transcribed spacer , microbiology and biotechnology , 18s ribosomal rna , rna , escherichia coli , ribosome

Structure and organization of two complete ribosomal RNA (rRNA) gene sets, rrnO and rrnA, were determined for the first time in Bacillus subtilis. They are located at the region of the replication origin of the chromosome. Each set constitutes a single operon of: two tandem promoters - leader sequence - 16S rRNA gene - Ile-tRNA gene - Ala-tRNA gene - 23S rRNA gene - 5S rRNA gene - termination signal. The first promoter (P1) of rrnO differs from that of rrnA in sequence and function. P1 of rrnO was used very little for transcription either in vivo or in vitro while P1 was predominantly used in rrnA. A putative transcript of the entire operon was determined and constructed into a secondary structure. Analysis of in vivo transcripts by S1 mapping revealed primary processing sites at the loop and stem structure of 16S rRNA in rrnO and rrnA. A unique sequence in the leader region of rrnO can be formed into a highly complexed secondary structure and affects processing of mature 16S rRNA. The sequences of the two spacer tRNA genes are highly conserved between B. subtilis and Escherichia coli.

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