Hop stunt viroid: molecular cloning and nucleotide sequence of the complete cDNA copy | Zendy

Takeshi Ohno | Zendy; Nobuhiko Takamatsu | Zendy; Tetsuo Meshi | Zendy; Yoshimi Okada | Zendy

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Hop stunt viroid: molecular cloning and nucleotide sequence of the complete cDNA copy

Author(s) -

Takeshi Ohno,

Nobuhiko Takamatsu,

Tetsuo Meshi,

Yoshimi Okada

Publication year - 1983

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/11.18.6185

Subject(s) - viroid , biology , complementary dna , nucleic acid sequence , rna , genetics , virology , microbiology and biotechnology , base pair , sequence (biology) , gene , complete sequence , genome

The complete cDNA of hop stunt viroid (HSV) has been cloned by the method of Okayama and Berg (Mol.Cell.Biol.2,161-170. (1982] and the complete nucleotide sequence has been established. The covalently closed circular single-stranded HSV RNA consists of 297 nucleotides. The secondary structure predicted for HSV contains 67% of its residues base-paired. The native HSV can possess an extended rod-like structure characteristic of viroids previously established. The central region of the native HSV has a similar structure to the conserved region found in all viroids sequenced so far except for avocado sunblotch viroid. The sequence homologous to the 5'-end of U1a RNA is also found in the sequence of HSV but not in the central conserved region.

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