Open AccessIsotation and characterisation of Dral, a type II restriction endonuclease recognisting a aequence containing only A:T basepairs, and inhibition of its activity by uv irradiation of substrate DNA
Author(s) -
Ian J. Purvis,
Bevan Moseley
Publication year - 1983
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/11.16.5467
Subject(s) - biology , dna , endonuclease , restriction enzyme , substrate (aquarium) , microbiology and biotechnology , biochemistry , enzyme , substrate specificity , genetics , biophysics , ecology
A type II restriction endonuclease, DraI, isolated from Deinococcus radiophilus ATCC 27603 recognises the palindromic hexanucleotide sequence (formula; see text) and cleaves it, as indicated by the arrows, to produce blunt-ended fragments. The yield of enzyme is 100 to 1000 times that of the only other known type II restriction endonuclease that recognises a sequence composed solely of A:T basepairs, the isoschizomer AhaIII (1). Ultraviolet irradiation of the DNA substrate at relatively low doses inhibits the activity of DraI by "protecting" the recognition sequence and this may be exploited to give control of partial digestion of DNA by DraI.