Conformationsl analysis of the trinucleoside diphosphate 3′d(A2′-5′A2′-5′A). An NMR and CD study | Zendy

Johannes Doornbos | Zendy; Ramamurthy Charubala | Zendy; Wolfgang Pfleiderer | Zendy; C. Altona | Zendy

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Conformationsl analysis of the trinucleoside diphosphate 3′d(A2′-5′A2′-5′A). An NMR and CD study

Author(s) -

Johannes Doornbos,

Ramamurthy Charubala,

Wolfgang Pfleiderer,

C. Altona

Publication year - 1983

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/11.13.4569

Subject(s) - trimer , ring (chemistry) , stacking , proton nmr , crystallography , circular dichroism , nuclear magnetic resonance spectroscopy , stereochemistry , proton , nuclear magnetic resonance , chemistry , physics , dimer , organic chemistry , quantum mechanics

A 500 MHz and 300 MHz NMR study of the trinucleoside diphosphate 3'd(A2'-5'A2'-5'A) is presented. In addition, circular dichroism is used to study base stacking in the title compound. The complete 1H-NMR spectral assignment of the sugar ring proton signals is given. Information about the sugar ring (N- or S-type conformation) and about the backbone geometry along C4'-C5' and C5'-O5' bonds is obtained from the NMR coupling constants. It is shown that the trimer mainly occurs in the N-N-N stacked state at low temperatures; the presence of a minor amount of N-N-S conformational sequence is indicated.

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