Cloning and expression of thestaphyiococcus aureusprotein A gene inEscheirchia Coli | Zendy

Clive J. Duggleby | Zendy; Susan Jones | Zendy

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Cloning and expression of thestaphyiococcus aureusprotein A gene inEscheirchia Coli

Author(s) -

Clive J. Duggleby,

Susan Jones

Publication year - 1983

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/11.10.3065

Subject(s) - biology , staphylococcus aureus , cloning (programming) , escherichia coli , gene , microbiology and biotechnology , blot , dna , molecular cloning , southern blot , strain (injury) , recombinant dna , gene expression , genetics , bacteria , anatomy , computer science , programming language

A gene bank of Staphylococcus aureus strain Cowan I was established using an E. coli HB101/pBR327 host-vector system. Recombinants expressing staphylococcal protein A (SPA) were detected using an IgG-binding assay. A 3.2 Kb DNA fragment directing the synthesis of SPA in E. coli was identified. SPA produced by E. coli was characterised in minicells and by Western blotting and double diffusion experiments.

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