Conservation of high efficiency promoter sequences inSaccharomyces cerevisiae | Zendy

Melanie J. Dobson | Zendy; Mick F. Tuite | Zendy; Nigel Roberts | Zendy; Alan J. Kingsman | Zendy; Susan M. Kingsman | Zendy; R.E. Perkins | Zendy; Stephen C. Conroy | Zendy; B. Dunbar | Zendy; Linda A. Fothergill | Zendy

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Conservation of high efficiency promoter sequences inSaccharomyces cerevisiae

Author(s) -

Melanie J. Dobson,

Mick F. Tuite,

Nigel Roberts,

Alan J. Kingsman,

Susan M. Kingsman,

R.E. Perkins,

Stephen C. Conroy,

B. Dunbar,

Linda A. Fothergill

Publication year - 1982

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/10.8.2625

Subject(s) - biology , saccharomyces cerevisiae , genetics , promoter , computational biology , yeast , gene , gene expression

The position of the yeast phosphoglycerate kinase (PGK) gene has been mapped on a 2.95kb Hind III fragment. We have determined the nucleotide sequence of the 5' flanking region and compared this sequence with those from 16 other yeast genes. PGK, like all other yeast genes has an adenine residue at position -3. It has two possible TATA boxes at positions -114 and -152 and a CAAT box at -129. In addition we have defined a structure at position -63 to -39 that is common to all yeast genes that encode an abundant RNA. This structure is a CT-rich block followed, about 10 nucleotides later, by the sequence CAAG.

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