On the cooperative and noncooperative binding of ethidium to DNA | Zendy

Stephen A. Winkle | Zendy; Leonard S. Rosenberg | Zendy; Thomas R. Krugh | Zendy

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On the cooperative and noncooperative binding of ethidium to DNA

Author(s) -

Stephen A. Winkle,

Leonard S. Rosenberg,

Thomas R. Krugh

Publication year - 1982

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/10.24.8211

Subject(s) - ethidium bromide , biology , dna , microbiology and biotechnology , biochemistry , biophysics

The equilibrium binding of ethidium bromide to native DNAs and to poly(dG-dC) X poly(dG-dC) has been studied by both phase partition and direct spectrophotometric techniques. The binding isotherms obtained from both experimental techniques show that ethidium binds in a cooperative manner to E. coli DNA. On the other hand, no evidence of cooperative binding was observed in the binding isotherms obtained with calf thymus, C. perfringens, M. lysodeikticus, or poly(dG-dC) X (dG-dC) under the experimental conditions used (0.1 M NaCl).

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