Effect of rifampicin on expression oflacZfused to promoters or terminators of theE.coli rpoBCoperon | Zendy

Kathy Howe | Zendy; Andrew J. Newman | Zendy; Ian Garner | Zendy; Anne E. Wallis | Zendy; Richard S. Hayward | Zendy

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Effect of rifampicin on expression oflacZfused to promoters or terminators of theE.coli rpoBCoperon

Author(s) -

Kathy Howe,

Andrew J. Newman,

Ian Garner,

Anne E. Wallis,

Richard S. Hayward

Publication year - 1982

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/10.22.7425

Subject(s) - biology , operon , rna polymerase , promoter , transcription (linguistics) , attenuator (electronics) , lac operon , gal operon , microbiology and biotechnology , gene , l arabinose operon , rna polymerase i , plasmid , polymerase , genetics , gene expression , rna , escherichia coli , linguistics , philosophy , physics , attenuation , optics

The genes encoding the beta and beta' subunits of RNA polymerase in E.coli lie downstream of at least two ribosomal protein genes in a single unit of transcription. Treatment of E.coli with rifampicin, under conditions producing partial inhibition of general RNA synthesis, can strongly stimulate transcription of the polymerase genes, while activating the neighbouring ribosomal genes only slightly. We have investigated the mechanism of this effect by fusing strong promoters, a weak internal promoter, and an attenuator of the polymerase operon to the lacZ gene, in derivatives of plasmid pMC81. Studies of these fusions confirm our conclusion, based on similar fusions to galK, that rifampicin can foster readthrough of transcriptional terminators. They also suggest the existence of extra terminators and anti-termination elements in the above transcription unit.

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