Hypoxanthine-guanine phosphoribosyltransferase genes of mouse and Chinese hamster: construction and sequence analysis of cDNA recombinants | Zendy

David Konecki | Zendy; John Brennand | Zendy; James C. Fuscoe | Zendy; C. Thomas Caskey | Zendy; A. Craig Chinault | Zendy

Open Access

Hypoxanthine-guanine phosphoribosyltransferase genes of mouse and Chinese hamster: construction and sequence analysis of cDNA recombinants

Author(s) -

David Konecki,

John Brennand,

James C. Fuscoe,

C. Thomas Caskey,

A. Craig Chinault

Publication year - 1982

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/10.21.6763

Subject(s) - biology , complementary dna , chinese hamster , microbiology and biotechnology , hypoxanthine guanine phosphoribosyltransferase , nucleic acid sequence , hypoxanthine phosphoribosyltransferase , phosphoribosyltransferase , gene , peptide sequence , coding region , hamster , sequence analysis , cdna library , hypoxanthine , genetics , biochemistry , dna , enzyme , mutant

Recombinant plasmids containing DNA inserts complementary to mRNA coding for hypoxanthine-guanine phosphoribosyltransferase (HPRT) from mouse and Chinese hamster cell lines have been isolated from cDNA libraries and characterized by DNA sequence analysis. A total of 1292 nucleotides of the mouse cDNA sequence and 1301 nucleotides of the Chinese hamster cDNA sequence has been determined. Each of these sequences includes an open reading frame of 654 nucleotides (218 amino acids) corresponding to the HPRT protein coding region. The deduced amino acid sequences for the mouse and Chinese hamster enzymes are presented and compared to that of human HPRT. At least 95% of the amino acids are conserved in the three species. In addition, we present evidence that two species of HPRT mRNA, which differ in the site of polyadenylation that is utilized during processing of the RNA transcripts, exist in Chinese hamster cells.

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