Regulation of transcription from tandem and convergent promoters | Zendy

Heidi Horowitz | Zendy; Terry Platt | Zendy

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Regulation of transcription from tandem and convergent promoters

Author(s) -

Heidi Horowitz,

Terry Platt

Publication year - 1982

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/10.18.5447

Subject(s) - promoter , biology , transcription (linguistics) , repressor , microbiology and biotechnology , rna polymerase ii , transcription factor , genetics , gene , gene expression , linguistics , philosophy

We have examined transcription on templates containing the trp and lac UV5 promoters arranged in tandem or opposing orientations. These studies have revealed that the strengths of the two promoters are comparable, though the lac UV5 promoter is much more sensitive to the level of initiating purine present. Kinetic experiments have shown that a polymerase molecule poised at the lac promoter, or a lac repressor molecule bound to the lac operator, can temporarily block a polymerase molecule initiated from the trp promoter, though transcription eventually continues through. In the convergent construct, transcription from the lac promoter is hindered only when initiation is suboptimal due to low purine concentrations.

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