Site directedin-vitroassembly of nucleosomes | Zendy

Stephanie Wittig | Zendy; Burghardt Wittig | Zendy

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Site directedin-vitroassembly of nucleosomes

Author(s) -

Stephanie Wittig,

Burghardt Wittig

Publication year - 1982

Publication title -

nucleic acids research

Language(s) - Uncategorized

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/10.12.3647

Subject(s) - biology , nucleosome , nucleoprotein , dna , histone , biophysics , in vitro , linker dna , microbiology and biotechnology , genetics , biochemistry

An in-vitro system is described allowing for the assembly of nucleosomes on preselected sites of a cloned tRNA gene. The system consists of a soluble nucleoprotein fraction, a histone source, and circular DNA containing a single stranded stretch (sssDNA). Nucleosomes assemble on the sssDNA, if the three components are incubated as a highly concentrated solution in the presence of the four deoxyribonucleotidetriphosphates and of ATP. The single stranded stretch is rendered double stranded during incubation. The middle axis of one assembled nucleosome always coincides roughly with the midpoint of the original single stranded DNA stretch.

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