Construction and expression in vivo of an internally deleted mouse α-fetoprotein gene: presence of a transcribed Alu-like repeat within the first intervening sequence | Zendy

Peter R. Young | Zendy; Richard Scott | Zendy; Dean H. Hamer | Zendy; Shirley M. Tilghman | Zendy

Open Access

Construction and expression in vivo of an internally deleted mouse α-fetoprotein gene: presence of a transcribed Alu-like repeat within the first intervening sequence

Author(s) -

Peter R. Young,

Richard Scott,

Dean H. Hamer,

Shirley M. Tilghman

Publication year - 1982

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/10.10.3099

Subject(s) - minigene , biology , exon , alu element , microbiology and biotechnology , exon trapping , polyadenylation , gene , nucleic acid sequence , genetics , rna splicing , intron , alternative splicing , gene expression , rna , genome , human genome

An alpha-fetoprotein (AFP) 'minigene' was constructed by joining the first two exons along with 0.9 kilobases of 5' flanking sequence of the mouse AFP gene to its last exon and 0.4 kilobases of 3' flanking sequence. This 'minigene' was tested for activity by inserting it into an SV40 vector and infecting African green monkey kidney cells. Correct initiation, termination, polyadenylation and splicing were observed. Furthermore a 220 nucleotide transcript was detected and mapped to a mouse Alu-like or B1 repeat on the opposite strand to that encoding the AFP gene in the first intervening sequence.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research