The thermal denaturatlon of DNA: average length and composition of denatured areas | Zendy

Anthony P. Russell | Zendy; David S. Holleman | Zendy

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The thermal denaturatlon of DNA: average length and composition of denatured areas

Author(s) -

Anthony P. Russell,

David S. Holleman

Publication year - 1974

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/1.8.959

Subject(s) - biology , dna , hydroxylapatite , melting curve analysis , nucleic acid denaturation , fractionation , nucleic acid thermodynamics , crystallography , biophysics , biochemistry , polymerase chain reaction , base sequence , chromatography , chemistry , enzyme , gene

A spectral study of melting curves of DNA ranging from 73 to 32% AT indicates that the base ratio of sequences melting within DNA are a linear function of temperature. A study of partially denatured DNA by electron microscopy, reversible renaturation and fractionation on hydroxylapatite suggests that the melting curve of DNA represents the melting of sequences which average 3-4 million daltons in length. These sequences appear to be a combination of two areas, one which is high in AT and denatures in the first three-quarters of the melting curve, and one which is high in GC and denatures in the final quarter. The length of these sequences appears to vary between 1.5-6 million daltons.

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