The mapping and ordering of fragments of SV40 DNA produced by restriction endonucleases | Zendy

K. N. Subramanian | Zendy; J. Pan | Zendy; S Zain | Zendy; S Weissman | Zendy

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The mapping and ordering of fragments of SV40 DNA produced by restriction endonucleases

Author(s) -

K. N. Subramanian,

J. Pan,

S Zain,

S Weissman

Publication year - 1974

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/1.6.727

Subject(s) - restriction enzyme , biology , cleavage (geology) , restriction fragment , dna , restriction map , ecori , microbiology and biotechnology , restriction site , genetics , base sequence , paleontology , fracture (geology)

SV40 DNA is cleaved by the Eco RII and Hae restriction endonucleases to give rise to two different sets of 16 fragments each. These fragments have been ordered by analysis of the products of redigestion of one set of fragments with another restriction enzyme. The cleavage sites have been precisely mapped based on length measurements of the products of each cleavage. This provides a convenient group of small DNA fragments suitable for sequence analysis investigation of the transcripts present in infected cells, or construction of deletion substitution variants of the virus.

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