Open AccessThe reactivity of the thiol groups of calf thymus deoxyribonucleohistone
Author(s) -
John E. Hyde,
I.O. Walker
Publication year - 1974
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/1.2.203
Subject(s) - dtnb , thiol , reactivity (psychology) , cysteine , biology , biochemistry , dna supercoil , reagent , stereochemistry , dna , chemistry , organic chemistry , enzyme , glutathione , medicine , dna replication , alternative medicine , pathology
The reactivities of the two cysteine thiol groups of calf thymus F3 histone were investigated using 5,5'-dithiobis-[2- nitrobenzoic acid], (DTNB). In isolated histone, both thiol groups were available for reaction. However, analysis of reaction profiles of native deoxyribonucleohistone, (DNH), in various solvent conditions, together with gel electrophoresis studies of DNH modified with DTNB, showed that only one of the thiol groups is normally modified by the reagent. If NaCl is present (above 1.OM) the other thiol group can also be modified. The reactivities of both groups were largely independent of the degree of DNH supercoiling and of the binding of F3 to the DNA.