Enzymatic synthesis of deoxyribo-oligonucleotides of defined sequence. Properties of the enzyme | Zendy

Shirley Gillam | Zendy; Michael J. Smith | Zendy

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Enzymatic synthesis of deoxyribo-oligonucleotides of defined sequence. Properties of the enzyme

Author(s) -

Shirley Gillam,

Michael J. Smith

Publication year - 1974

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/1.12.1631

Subject(s) - polynucleotide phosphorylase , biology , deoxyribonucleotide , enzyme , biochemistry , oligonucleotide , escherichia coli , nucleotide , microbiology and biotechnology , gel electrophoresis , purine nucleoside phosphorylase , dna , purine , gene

A modified purification is described for an enzyme, from Escherichiacoli B, which polymerizes deoxyribonucleoside-5' diphosphates. Under appropriate conditions, the enzyme will add a single deoxyribonucleotide residue to a deoxyribo-oligonucleotide primer. At all stages, the enzyme activity copurified with the activity which will polymerize adenosine-5' diphosphate (polynucleotide phosphorylase). Studies of heat stability, the effect of various temperatures of reaction and of disc gel electrophoresis failed to provide evidence that the two activities are separable.

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