The effects of Atm haploinsufficiency on mutation rate in the mouse germ line and somatic tissue | Zendy

A. K. Ahuja | Zendy; Ruth Barber | Zendy; Robert J. Hardwick | Zendy; Michael M. Weil | Zendy; Paula C. Genik | Zendy; David J. Brenner | Zendy; Yuri E. Dubrova | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

The effects of Atm haploinsufficiency on mutation rate in the mouse germ line and somatic tissue

Author(s) -

A. K. Ahuja,

Ruth Barber,

Robert J. Hardwick,

Michael M. Weil,

Paula C. Genik,

David J. Brenner,

Yuri E. Dubrova

Publication year - 2008

Publication title -

mutagenesis

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.723

H-Index - 91

eISSN - 1464-3804

pISSN - 0267-8357

DOI - 10.1093/mutage/gen028

Subject(s) - haploinsufficiency , somatic cell , mutation frequency , bone marrow , sperm , microbiology and biotechnology , biology , mutation , germline mutation , mutation rate , andrology , genetics , immunology , phenotype , medicine , gene

Using single-molecule polymerase chain reaction, the frequency of spontaneous and radiation-induced mutation at an expanded simple tandem repeat (ESTR) locus was studied in DNA samples extracted from sperm and bone marrow of Atm knockout (Atm(+/-)) heterozygous male mice. The frequency of spontaneous mutation in sperm and bone marrow in Atm(+/-) males did not significantly differ from that in wild-type BALB/c mice. Acute exposure to 1 Gy of gamma-rays did not affect ESTR mutation frequency in bone marrow and resulted in similar increases in sperm samples taken from Atm(+/-) and BALB/c males. Taken together, these results suggest that the Atm haploinsufficiency analysed in our study does not affect spontaneous and radiation-induced ESTR mutation frequency in mice.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research