Induction of micronuclei and sister chromatid exchange in mouse splenocytes after exposure to the butadiene metabolite 3, 4-epoxy-1-butene | Zendy

G. Stephanou | Zendy; C. Andrianopoulos | Zendy; Dimitris Vlastos | Zendy; N.A. Demopoulos | Zendy; Antonella Russo | Zendy

Open Access

Induction of micronuclei and sister chromatid exchange in mouse splenocytes after exposure to the butadiene metabolite 3, 4-epoxy-1-butene

Author(s) -

G. Stephanou,

C. Andrianopoulos,

Dimitris Vlastos,

N.A. Demopoulos,

Antonella Russo

Publication year - 1997

Publication title -

mutagenesis

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.723

H-Index - 91

eISSN - 1464-3804

pISSN - 0267-8357

DOI - 10.1093/mutage/12.6.425

Subject(s) - sister chromatid exchange , micronucleus test , micronucleus , chemistry , metabolite , splenocyte , in vivo , somatic cell , microbiology and biotechnology , biochemistry , toxicity , biology , genetics , in vitro , organic chemistry , gene

3,4-Epoxy-1-butene (EB) is one of the main metabolites of 1,3 butadiene, a widely used industrial chemical. The mutagenic potential of 1,3 butadiene and its metabolites have been studied in different test systems. In this work the genotoxic effects of EB were studied by estimating micronuclei (MN) and sister chromatid exchange (SCE) frequencies in stimulated mouse splenocytes. Mice were treated in vivo with various doses of EB (24.4, 48.8 and 73.2 mg/kg). The antikinetochore antibody technique (CREST) was also applied to MN in cytokinesis blocked cells to investigate any possible aneugenic effect. Both MN and SCE frequencies increased after EB treatment. The induced MN resulted mainly from acentric fragments but a weak aneugenic effect was found as well. Cytotoxic effects of EB were observed at the highest dose. The above results, in combination with others on the effect of 1,3 butadiene and its metabolites in somatic and germ cells of mouse and rat as well as in somatic human cells, form a part of the information needed for application of the parallelogram approach and extrapolation to human risk.

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