Genotoxicity of tetrodotoxin from puffer fish tested in root meristem cells of Allium cepa L. | Zendy

Samanta S. Khora | Zendy; Kamal K. Panda | Zendy; Brahma B. Panda | Zendy

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Genotoxicity of tetrodotoxin from puffer fish tested in root meristem cells of Allium cepa L.

Author(s) -

Samanta S. Khora,

Kamal K. Panda,

Brahma B. Panda

Publication year - 1997

Publication title -

mutagenesis

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.723

H-Index - 91

eISSN - 1464-3804

pISSN - 0267-8357

DOI - 10.1093/mutage/12.4.265

Subject(s) - genotoxicity , micronucleus test , mitotic index , allium , metaphase , clastogen , sister chromatid exchange , sister chromatids , biology , micronucleus , mitosis , meristem , microbiology and biotechnology , chemistry , genetics , botany , dna , toxicity , chromosome , gene , organic chemistry

Tetrodotoxin (TTX) extracted and purified from puffer fish Arothron nigropunctatus was tested for genotoxicity employing the root meristem cells of Allium cepa as the assay system. The genotoxicity endpoints investigated were mitotic index (MI), meta-anaphases with spindle aberrations, interphases with micronuclei (MNC) and sister chromatid exchanges (SCE) in metaphase chromosomes. The results demonstrated that TTX inhibited mitosis at concentrations of > or = 30 microM as evident by the fall of MI, but failed to induce MNC at significant levels at any of the concentrations tested (10-100 microM). TTX was thus proved to be neither clastogenic nor aneugenic in the present study. It was, however, noteworthy that TTX at far lower concentrations, 0.1-5.0 microM, significantly enhanced the frequencies of SCE which indicated possible interference of the toxin in DNA replication and repair.

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