Lowering extracellular calcium content protects cells from arsenite-induced killing and micronuclei formation | Zendy

Yee-Chien Liu | Zendy; Haimei Huang | Zendy

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Lowering extracellular calcium content protects cells from arsenite-induced killing and micronuclei formation

Author(s) -

Yee-Chien Liu,

Haimei Huang

Publication year - 1996

Publication title -

mutagenesis

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.723

H-Index - 91

eISSN - 1464-3804

pISSN - 0267-8357

DOI - 10.1093/mutage/11.1.75

Subject(s) - calcium , extracellular , verapamil , egta , arsenite , chemistry , calcium in biology , cytotoxicity , intracellular , calcium metabolism , calcium channel blocker , nitrendipine , micronucleus test , biochemistry , pharmacology , biology , toxicity , in vitro , arsenic , organic chemistry

The present study demonstrated that calcium ions were accumulated in nuclei of Chinese hamster ovary (CHO)-K1 cells after arsenite treatment. This process was enhanced by verapamil (a calcium channel blocker). Verapamil also significantly increased the cytotoxic effects of arsenite. In contrast, ethylene glycol-bis[beta-aminoethylether] N,N, N1,N1,-tetraacetic acid (EGTA, a calcium-specific chelator), or calcium-free conditions significantly reduced the cytotoxicity or arsenite. Similarly, the strategy of lowering extracellular calcium concentration by modifying the medium or lowering intracellular calcium concentration by administration of the intracellular calcium chelator quin 2, protected cells from arsenite-induced micronuclei formation. These data indicate that the disturbances in intracellular calcium homeostasis maybe involved in arsenite-induced cytotoxicity and micronuclei formation.

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