Open AccessDetection of human novel developmental genes in cDNA derived from replicate individual preimplantation embryos
Author(s) -
Cathy Holding
Publication year - 2000
Publication title -
molecular human reproduction
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.143
H-Index - 122
eISSN - 1460-2407
pISSN - 1360-9947
DOI - 10.1093/molehr/6.9.801
Subject(s) - biology , complementary dna , embryo , replicate , blastocyst , differential display , gene , reverse transcriptase , gene expression , genetics , reverse transcription polymerase chain reaction , polymerase chain reaction , microbiology and biotechnology , embryogenesis , statistics , mathematics
We have constructed amplified cDNA preparations from replicate samples of human oocytes and individual preimplantation embryos. Differential display of the cDNA preparations shows disparate patterns of gene expression in the individual embryos at all stages of preimplantation development. The variation in patterns of genes expressed is in part due to the low starting cell number undergoing the reverse transcription-polymerase chain reaction (RT-PCR) step in the preparation of the amplified cDNAs. Despite this variability, the use of replicate embryo samples makes it possible to identify and isolate human genes specifically expressed at the different stages of human preimplantation development from the unfertilized oocyte to the blastocyst stage.
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