Loop-Mediated Isothermal Amplification on Crude DNA as a Point-of-Care Test for the Diagnosis of Mycoplasma-Related Vaginitis During Early Pregnancy | Zendy

Yichao Wang | Zendy; Bumei Zhang | Zendy; Yan Sun | Zendy; Yunde Liu | Zendy; Yajun Gu | Zendy

Open Access

Loop-Mediated Isothermal Amplification on Crude DNA as a Point-of-Care Test for the Diagnosis of Mycoplasma-Related Vaginitis During Early Pregnancy

Author(s) -

Yichao Wang,

Bumei Zhang,

Yan Sun,

Yunde Liu,

Yajun Gu

Publication year - 2017

Publication title -

laboratory medicine

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.332

H-Index - 28

eISSN - 1943-7730

pISSN - 0007-5027

DOI - 10.1093/labmed/lmx063

Subject(s) - loop mediated isothermal amplification , vaginitis , point of care testing , medicine , isothermal process , mycoplasma , obstetrics , pregnancy , gynecology , dna , biology , microbiology and biotechnology , physics , genetics , immunology , thermodynamics

Mycoplasma-related vaginitis gradually has been growing as a threat in adults-genitourinary infection contributes to funisitis, spontaneous abortion, and low birth weight. Until now, use of loop-mediated isothermal amplification (LAMP) to detect Ureaplasma urealyticum (UU), Mycoplasma hominis (MH), or Mycoplasma genitalium (MG) has been reported by some researchers. However, previous studies focused on purified DNA as the template for LAMP assay, which is usually extracted via commercial kit.

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