Leukocyte Dynamics Influence Reference Gene Stability in Whole Blood: Data-Driven qRT-PCR Normalization Is a Robust Alternative for Measurement of Transcriptional Biomarkers | Zendy

Grant C. O’Connell | Zendy; Madison B Treadway | Zendy; Ashley B. Petrone | Zendy; Connie S. Tennant | Zendy; Noelle Lucke-Wold | Zendy; Paul D. Chantler | Zendy; Taura L. Barr | Zendy

Open Access

Leukocyte Dynamics Influence Reference Gene Stability in Whole Blood: Data-Driven qRT-PCR Normalization Is a Robust Alternative for Measurement of Transcriptional Biomarkers

Author(s) -

Grant C. O’Connell,

Madison B Treadway,

Ashley B. Petrone,

Connie S. Tennant,

Noelle Lucke-Wold,

Paul D. Chantler,

Taura L. Barr

Publication year - 2017

Publication title -

laboratory medicine

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.332

H-Index - 28

eISSN - 1943-7730

pISSN - 0007-5027

DOI - 10.1093/labmed/lmx035

Subject(s) - reference genes , normalization (sociology) , gene expression , gene , gene expression profiling , biology , white blood cell , whole blood , real time polymerase chain reaction , computational biology , bioinformatics , immunology , genetics , sociology , anthropology

The use of reference genes for normalization of whole blood qRT-PCR data may be problematic in conditions such as stroke which induce alterations in white blood cell differential. In this study, we assessed the influence of stroke on the stability of commonly employed reference genes, and we evaluated data-driven normalization as an alternative.

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