Association Between Presence of Virulence Genes and Antibiotic Resistance in ClinicalKlebsiella PneumoniaeIsolates
Author(s) -
Safoura Derakhshan,
Shahin Najar Peerayeh,
Bita Bakhshi
Publication year - 2016
Publication title -
laboratory medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.332
H-Index - 28
eISSN - 1943-7730
pISSN - 0007-5027
DOI - 10.1093/labmed/lmw030
Subject(s) - integron , klebsiella pneumoniae , virulence , microbiology and biotechnology , biology , gene , polymerase chain reaction , antibiotic resistance , antibiotics , agar diffusion test , genetics , escherichia coli
To investigate the presence of rmpA and wcaG virulence genes and Class 1, 2, and 3 integrons, and to evaluate a relationship between antibiotic resistance and virulence in Klebsiella pneumoniae METHODS: We collected a total of 200 K. pneumoniae isolates from hospitals in Tehran, Iran. Antibiotic susceptibility was determined using the disk diffusion method. The extended-spectrum β-lactamase (ESBL) producers were detected using the combination disk method. We detected the rmpA and wcaG genes and class 1, 2, and 3 integrons via polymerase chain reaction (PCR). The χ 2 est was used for statistical evaluation.
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