A Carotenoid Cleavage Dioxygenase from Vitis vinifera L.: functional characterization and expression during grape berry development in relation to C13-norisoprenoid accumulation | Zendy

Sandrine Mathieu | Zendy; Nancy Terrier | Zendy; Jérôme Procureur | Zendy; Frédéric Bigey | Zendy; Ziya Günata | Zendy

Open Access

A Carotenoid Cleavage Dioxygenase from Vitis vinifera L.: functional characterization and expression during grape berry development in relation to C13-norisoprenoid accumulation

Author(s) -

Sandrine Mathieu,

Nancy Terrier,

Jérôme Procureur,

Frédéric Bigey,

Ziya Günata

Publication year - 2005

Publication title -

journal of experimental botany

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.616

H-Index - 242

eISSN - 1460-2431

pISSN - 0022-0957

DOI - 10.1093/jxb/eri265

Subject(s) - vitis vinifera , berry , carotenoid , dioxygenase , cleavage (geology) , chemistry , botany , biology , biochemistry , gene , paleontology , fracture (geology)

A potential Carotenoid Cleavage Dioxygenase (CCD) gene was identified among a Vitis vinifera L. EST collection and a full-length cDNA (VvCCD1) was isolated. Recombinant expression of VvCCD1 confirmed that the gene encoded a functional CCD. Experimental evidence was obtained that VvCCD1 cleaves zeaxanthin symmetrically yielding 3-hydroxy-beta-ionone, a C(13)-norisoprenoidic compound, and a C(14)-dialdehyde. Expression of the gene was studied by real-time PCR at different developmental stages of grape berries from Muscat of Alexandria and Shiraz cultivars. A significant induction of the gene expression approaching véraison was observed in both cultivars. In parallel, the C(13)-norisoprenoid level increased from véraison to maturity in both cultivars.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research