Vacuolar system distribution in Arabidopsis tissues, visualized using GFP fusion proteins | Zendy

Rudolf Flückiger | Zendy

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Vacuolar system distribution in Arabidopsis tissues, visualized using GFP fusion proteins

Author(s) -

Rudolf Flückiger

Publication year - 2003

Publication title -

journal of experimental botany

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.616

H-Index - 242

eISSN - 1460-2431

pISSN - 0022-0957

DOI - 10.1093/jxb/erg160

Subject(s) - green fluorescent protein , arabidopsis , vacuole , microbiology and biotechnology , fusion protein , protein subcellular localization prediction , biology , subcellular localization , arabidopsis thaliana , gene , cytoplasm , biochemistry , mutant , recombinant dna

Green fluorescent protein (GFP) allows the direct visualization of gene expression and the subcellular localization of fusion proteins in living cells. The localization of different GFP fusion proteins in the secretory system was studied in stably transformed Arabidopsis plants cv. Wassilewskaja. Secreted GFP (SGFP) and GFP retained in the ER (GFP-KDEL) confirmed patterns already known, but two vacuolar GFPs (GFP-Chi and Aleu-GFP) labelled the Arabidopsis vacuolar system for the first time, the organization of which appears to depend on cell differentiation. GFP stability in the vacuoles may depend on pH or degradation, but these vacuolar markers can, nevertheless, be used as a tool for physiological studies making these plants suitable for mutagenesis and gene-tagging experiments.

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