Zea mays ZmMybst1 cDNA, encodes a single Myb-repeat protein with the VASHAQKYF motif | Zendy

Inderjit S. Mercy | Zendy; Robert Meeley | Zendy; Scott E. Nichols | Zendy; OddArne Olsen | Zendy

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Zea mays ZmMybst1 cDNA, encodes a single Myb-repeat protein with the VASHAQKYF motif

Author(s) -

Inderjit S. Mercy,

Robert Meeley,

Scott E. Nichols,

OddArne Olsen

Publication year - 2003

Publication title -

journal of experimental botany

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.616

H-Index - 242

eISSN - 1460-2431

pISSN - 0022-0957

DOI - 10.1093/jxb/erg118

Subject(s) - complementary dna , endosperm , aleurone , biology , gene , myb , northern blot , microbiology and biotechnology , intron , genetics , gene expression

A cDNA clone from a 4 DAP dissected maize embryo sac encoding a novel Zea mays single-repeat Myb protein is reported here. This full-length cDNA contains an ORF of 948 bp. The gene ZmMybst1 contains two introns (1166 and 706 bp) and is a single copy gene. The ZmMybst1 protein shares high sequence identity with the potato Mybst1 protein (58%). Northern blot, RT-PCR and electronic northern analysis shows that ZmMybst1 is expressed in endosperm between 4 and 30 DAP, coinciding with the period of aleurone cell differentiation and development.

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