Isolation and characterization of the orchid cytokinin oxidase DSCKX1 promoter | Zendy

Shuhua Yang | Zendy; Hao Yu | Zendy; Chong Jin Goh | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Isolation and characterization of the orchid cytokinin oxidase DSCKX1 promoter

Author(s) -

Shuhua Yang,

Hao Yu,

Chong Jin Goh

Publication year - 2002

Publication title -

journal of experimental botany

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.616

H-Index - 242

eISSN - 1460-2431

pISSN - 0022-0957

DOI - 10.1093/jxb/erf055

Subject(s) - cytokinin , gene , biology , arabidopsis , regulatory sequence , transformation (genetics) , coding region , genetics , oxidase test , mutant , regulation of gene expression , biochemistry , auxin , enzyme

The orchid DSCKX1 is a new member of the cytokinin oxidase gene family, which catalyses the degradation of cytokinins bearing unsaturated isoprenoid side chains. A 3.7 kb fragment upstream of the DSCKX1 coding region was isolated, sequenced and characterized by deletion analysis of DSCKX1::beta-glucuronidase gene fusions using transient orchid and stable Arabidopsis transformation systems. Functional analysis of 5' deletions defined the 5'-upstream region that directs the expression in distinct tissues. Regulatory elements affecting the cytokinin induction of the DSCKX1 gene have also been delineated

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research