Molecular cloning and characterization of plant genes encoding novel peroxisomal molybdoenzymes of the sulphite oxidase family | Zendy

Tatsuo Nakamura | Zendy

Open Access

Molecular cloning and characterization of plant genes encoding novel peroxisomal molybdoenzymes of the sulphite oxidase family

Author(s) -

Tatsuo Nakamura

Publication year - 2002

Publication title -

journal of experimental botany

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.616

H-Index - 242

eISSN - 1460-2431

pISSN - 0022-0957

DOI - 10.1093/jxb/erf042

Subject(s) - peroxisome , gene , cloning (programming) , molecular cloning , biology , genetics , biochemistry , complementary dna , computer science , programming language

The Arabidopsis AtMCP and rice OsMCP genes which encode proteins highly homologous to molybdoenzymes of the sulphite oxidase family were isolated and characterized. Both proteins seemed to possess only a molybdenum cofactor as the redox centre, unlike all the other eukaryotic molybdoenzymes. Putative MCP orthologues were identified in 17 plant species, indicating that MCPs are widely distributed over the plant kingdom [corrected]. An analysis using a green fluorescent protein fusion showed that AtMCP possesses a peroxisomal targeting signal at its C-terminus. Putative peroxisomal targeting signals were also found in all plant MCPs, suggesting the existence of a new redox pathway in this organelle.

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