Establishment of a reproducible tissue culture system for the induction of Arabidopsis somatic embryos | Zendy

Miho Ikeda | Zendy

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Establishment of a reproducible tissue culture system for the induction of Arabidopsis somatic embryos

Author(s) -

Miho Ikeda

Publication year - 2002

Publication title -

journal of experimental botany

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.616

H-Index - 242

eISSN - 1460-2431

pISSN - 0022-0957

DOI - 10.1093/jxb/erf006

Subject(s) - somatic embryogenesis , somatic cell , abscisic acid , embryo , biology , arabidopsis , microbiology and biotechnology , embryogenesis , tissue culture , gene , genetics , mutant , in vitro

Somatic embryogenesis is an example of totipotency and is used as a model system for studying embryogenesis. A reproducible tissue culture system was established for the large-scale induction of Arabidopsis somatic embryos. The method allows maintenance of high embryogenic competence over a one-year period. Using this tissue culture system, the expression of embryo-specific genes (ABI3, LEC1, FUS3) was detected in embryogenic cells and somatic embryos. Exogenous application of abscisic acid enhanced the expression of some late-embryogenesis-abundant (LEA) protein genes in somatic embryos. The experiments show that the method can be used to obtain sufficient amounts of embryogenic material for basic molecular analyses.

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