Cloning an iron-regulated metal transporter from rice | Zendy

N. Bughio | Zendy; Hirotaka Yamaguchi | Zendy; Naoko K. Nishizawa | Zendy; Hiromi Nakanishi | Zendy; Satoshi Mori | Zendy

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Cloning an iron-regulated metal transporter from rice

Author(s) -

N. Bughio,

Hirotaka Yamaguchi,

Naoko K. Nishizawa,

Hiromi Nakanishi,

Satoshi Mori

Publication year - 2002

Publication title -

journal of experimental botany

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.616

H-Index - 242

eISSN - 1460-2431

pISSN - 0022-0957

DOI - 10.1093/jxb/erf004

Subject(s) - complementary dna , clone (java method) , cloning (programming) , northern blot , biology , gene , transporter , cdna library , microbiology and biotechnology , arabidopsis , yeast , mutant , southern blot , arabidopsis thaliana , molecular cloning , genetics , computer science , programming language

Rice cDNA and genomic libraries were screened in order to clone an Fe(II) transporter gene. A cDNA clone highly homologous to the Arabidopsis Fe(II) transporter gene IRT1 was isolated from Fe-deficient rice roots. The cDNA clone was named OsIRT1. A genomic clone corresponding to the cDNA was also obtained, sequenced and analysed. When expressed in yeast cells, OsIRT1 cDNA reversed the growth defects of the yeast iron-uptake mutant. Northern blot analysis revealed that OsIRT1 mRNA was predominantly expressed in roots and was induced by Fe- and Cu-deficiency. This suggests that OsIRT1 is a functional metal transporter for iron, and is actively engaged in Fe uptake from soils, especially under limiting conditions.

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