Comparison of Whole-Genome Sequencing and Molecular-Epidemiological Techniques forClostridium difficileStrain Typing | Zendy

Samuel R. Dominguez | Zendy; Lydia Anderson | Zendy; Cassandra V. Kotter | Zendy; Cynthia A. Littlehorn | Zendy; Lesley E. Arms | Zendy; Elaine Dowell | Zendy; James K. Todd | Zendy; Daniel N. Frank | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Comparison of Whole-Genome Sequencing and Molecular-Epidemiological Techniques forClostridium difficileStrain Typing

Author(s) -

Samuel R. Dominguez,

Lydia Anderson,

Cassandra V. Kotter,

Cynthia A. Littlehorn,

Lesley E. Arms,

Elaine Dowell,

James K. Todd,

Daniel N. Frank

Publication year - 2015

Publication title -

journal of the pediatric infectious diseases society

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.269

H-Index - 31

eISSN - 2048-7207

pISSN - 2048-7193

DOI - 10.1093/jpids/piv020

Subject(s) - pulsed field gel electrophoresis , clostridium difficile , whole genome sequencing , typing , polymerase chain reaction , dna sequencing , strain (injury) , genome , microbiology and biotechnology , molecular epidemiology , biology , genetics , medicine , gene , genotype , anatomy , antibiotics

We analyzed in parallel 27 pediatric Clostridium difficile isolates by repetitive sequence-based polymerase chain reaction (RepPCR), pulsed-field gel electrophoresis (PFGE), and whole-genome next-generation sequencing. Next-generation sequencing distinguished 3 groups of isolates that were indistinguishable by RepPCR and 1 isolate that clustered in the same PFGE group as other isolates.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research