Use of ionic liquid for X-ray micro-CT specimen preparation of imbibed seeds | Zendy

Daisuke Yamauchi | Zendy; Aki Fukuda | Zendy; Tomonori Nakai | Zendy; Ichirou Karahara | Zendy; Miyuki Takeuchi | Zendy; Daisuke Tamaoki | Zendy; Tetsuya Tsuda | Zendy; Katsuhiko Tsunashima | Zendy; Susumu Kuwabata | Zendy; Masato Hoshino | Zendy; Kentaro Uesugi | Zendy; Akihisa Takeuchi | Zendy; Yoshio Suzuki | Zendy; Yoshinobu Mineyuki | Zendy

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Use of ionic liquid for X-ray micro-CT specimen preparation of imbibed seeds

Author(s) -

Daisuke Yamauchi,

Aki Fukuda,

Tomonori Nakai,

Ichirou Karahara,

Miyuki Takeuchi,

Daisuke Tamaoki,

Tetsuya Tsuda,

Katsuhiko Tsunashima,

Susumu Kuwabata,

Masato Hoshino,

Kentaro Uesugi,

Akihisa Takeuchi,

Yoshio Suzuki,

Yoshinobu Mineyuki

Publication year - 2018

Publication title -

microscopy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.545

H-Index - 52

eISSN - 2050-5701

pISSN - 2050-5698

DOI - 10.1093/jmicro/dfy130

Subject(s) - ionic liquid , osmium tetroxide , materials science , shrinkage , fixation (population genetics) , x ray , osmium , biomedical engineering , ionic bonding , chemical engineering , chemistry , composite material , biochemistry , ion , medicine , organic chemistry , optics , electron microscope , physics , ruthenium , gene , engineering , catalysis

X-ray micro-CT is one of the most useful techniques to examine 3D cellular architecture inside dry seeds. However, the examination of imbibed seeds is difficult because immersion in water causes a decline in the image quality. Here, we examined the use of ionic liquids for specimen preparation of chemically fixed imbibed seeds of Arabidopsis. We found that treatment with high concentrations of ionic liquids after osmium tetroxide fixation helped not only to prevent the structural damage caused by seed shrinkage, but also to preserve the image quality. Under these conditions, the cellular architecture of seeds was also well maintained.

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