Detection of Babesia microti DNA in Ixodes scapularis (Acari: Ixodidae) by Use of Chelex 100 Resin and Polymerase Chain Reaction | Zendy

Charles Thill | Zendy; P. Bryon Backenson | Zendy; Melissa A. Prusinski | Zendy; Sarah Kogut | Zendy; JoonHak Lee | Zendy; James L. Coleman | Zendy

Open Access

Detection of Babesia microti DNA in Ixodes scapularis (Acari: Ixodidae) by Use of Chelex 100 Resin and Polymerase Chain Reaction

Author(s) -

Charles Thill,

P. Bryon Backenson,

Melissa A. Prusinski,

Sarah Kogut,

JoonHak Lee,

James L. Coleman

Publication year - 2005

Publication title -

journal of medical entomology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.866

H-Index - 99

eISSN - 1938-2928

pISSN - 0022-2585

DOI - 10.1093/jmedent/42.4.694

Subject(s) - ixodes scapularis , ixodidae , biology , babesia , polymerase chain reaction , acari , babesiosis , ixodes , dna extraction , parasite hosting , parasitiformes , virology , microbiology and biotechnology , tick , zoology , genetics , gene , world wide web , computer science

A rapid and sensitive protocol using Chelex 100 resin for DNA extraction and the polymerase chain reaction for detection of Babesia microti Franca within its vector Ixodes scapularis Say is described. The level of detection of this protocol was determined to be the approximate equivalent of one parasite.

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