Molecular cloning and nucleotide sequence of CYP6BF1 from the diamondback moth, Plutella xylostella | Zendy

Hongshan Li | Zendy; Dai Hua-guo | Zendy; Hui Wei | Zendy

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Molecular cloning and nucleotide sequence of CYP6BF1 from the diamondback moth, Plutella xylostella

Author(s) -

Hongshan Li,

Dai Hua-guo,

Hui Wei

Publication year - 2005

Publication title -

journal of insect science

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.551

H-Index - 49

ISSN - 1536-2442

DOI - 10.1093/jis/5.1.45

Subject(s) - plutella , diamondback moth , biology , open reading frame , genbank , accession number (library science) , lepidoptera genitalia , complementary dna , genetics , nucleic acid sequence , gene , microbiology and biotechnology , peptide sequence , botany

A novel cDNA clong encoding a cytochrome P450 was screened from the insecticide-susceptible strain of Plutella xylostella (L.) (Lepidoptera:Yponomeutidae). The nucleotide sequence of the clone, designated CYP6BF1, was determined. This is the first full-length sequence of the CYP6 family from Plutella xylostella (L.). The cDNA is 1661bp in length and contains an open reading frame from base pairs 26 to 1570, encoding a protein of 514 amino acid residues. It is similar to the other insect P450s in gene family 6, including CYP6AE1 from Depressaria pastinacella, (46%). The GenBank accession number is AY971374.

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