Localization and Characterization of 170 BAC-Derived Clones and Mapping of 94 Microsatellites in the Hessian Fly | Zendy

Brandon J. Schemerhorn | Zendy; Charles F. Crane | Zendy; Philip K. Morton | Zendy; R. L. Aggarwal | Zendy; Thiago Romanos Benatti | Zendy

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Localization and Characterization of 170 BAC-Derived Clones and Mapping of 94 Microsatellites in the Hessian Fly

Author(s) -

Brandon J. Schemerhorn,

Charles F. Crane,

Philip K. Morton,

R. L. Aggarwal,

Thiago Romanos Benatti

Publication year - 2009

Publication title -

journal of heredity

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.99

H-Index - 92

eISSN - 1471-8505

pISSN - 0022-1503

DOI - 10.1093/jhered/esp045

Subject(s) - biology , polytene chromosome , microsatellite , bacterial artificial chromosome , genetics , chromosome , population , genetic diversity , genomic library , gene mapping , evolutionary biology , genome , gene , peptide sequence , sociology , allele , demography

Ninety-four microsatellites from enriched genomic libraries of Hessian fly (Hf, Mayetiola destructor [Say]) were localized to 170 cognate clones in an Hf bacterial artificial chromosome (BAC) library. These microsatellite-positive BAC clones were physically mapped to polytene chromosomes by fluorescent in situ hybridization. The mapped microsatellite loci can be used to study the genetic diversity and population structure of Hf.

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