Base Composition of Coffea AFLP Sequences and Their Conservation Within the Genus | Zendy

Valérie Poncet | Zendy; Perla Hamon | Zendy; M.-B. Sauvage de Saint Marc | Zendy; Thomas Bernard | Zendy; Serge Hamon | Zendy; Michel Noirot | Zendy

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Base Composition of Coffea AFLP Sequences and Their Conservation Within the Genus

Author(s) -

Valérie Poncet,

Perla Hamon,

M.-B. Sauvage de Saint Marc,

Thomas Bernard,

Serge Hamon,

Michel Noirot

Publication year - 2004

Publication title -

journal of heredity

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.99

H-Index - 92

eISSN - 1471-8505

pISSN - 0022-1503

DOI - 10.1093/jhered/esi013

Subject(s) - amplified fragment length polymorphism , biology , genetics , coffea , genetic diversity , coffea arabica , evolutionary biology , coding region , botany , gene , population , demography , sociology

Amplified fragment length polymorphism (AFLP) is often used for genetic mapping and diversity analysis, but very little information is currently available on their sequence characteristics. Species-specific sequences were analyzed from a single Coffea genome (Coffea pseudozanguebariae) associated with clustered or nonclustered AFLP loci of known genetic position. Compared with the expressed sequence tag (EST) sequence composition, their AT content exhibited a bimodal distribution with AT-poor sequences corresponding mainly to putative coding sequences. AT-rich sequences, apart from the EST distribution, were usually clustered on the genetic map and might correspond to noncoding sequences. Conversion of these AFLP markers into sequence-characterized amplified region (SCAR) anchor markers allowed us to assess sequence conservation within Coffea species with respect to species relatedness.

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