A Minisatellite with Fold-Back Structure is Included in the 5'-Flanking Region of the Adh Gene of Scaptodrosophila lebanonensis | Zendy

Dorcas J. Orengo | Zendy; Montserrat Papaceit | Zendy; E. Juan | Zendy

Open Access

A Minisatellite with Fold-Back Structure is Included in the 5'-Flanking Region of the Adh Gene of Scaptodrosophila lebanonensis

Author(s) -

Dorcas J. Orengo,

Montserrat Papaceit,

E. Juan

Publication year - 2004

Publication title -

journal of heredity

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.99

H-Index - 92

eISSN - 1471-8505

pISSN - 0022-1503

DOI - 10.1093/jhered/esh005

Subject(s) - biology , minisatellite , flanking maneuver , 5' flanking region , genetics , fold (higher order function) , gene , microbiology and biotechnology , allele , gene expression , microsatellite , mechanical engineering , history , promoter , archaeology , engineering

A tandem repetitive sequence with a repeat unit of 12 bp has been found 1.3 kb upstream of the Adh gene of Scaptodrosophila lebanonensis. This repetitive sequence extends over 4.3 kb and consists of two inverted arrays (a fold-back segment). The repeated unit with a consensus sequence GAATACAGAATA is highly conserved and the nucleotide substitutions are not distributed randomly among the 12 bp. In situ hybridization in S. lebanonensis polytene chromosomes revealed two signals, one at the 60A section, the Adh locus, and a second site in the same chromosome at the 60C section close to the telomere. This same pattern of hybridization is obtained in all the analyzed strains including the subspecies S. lebanonensis casteeli. The minisatellite sequence accounts for about 0.03-0.04% of the S. lebanonensis genome and showed intraspecific variability in tandem repeat numbers. Possible functions of this sequence are discussed.

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