Open AccessrDNA (18S-28S and 5S) Colocalization and Linkage Between Ribosomal Genes and (TTAGGG)n Telomeric Sequence in the Earthworm, Octodrilus complanatus (Annelida: Oligochaeta: Lumbricidae), Revealed by Single- and Double-Color FISH
Author(s) -
R. Vitturi
Publication year - 2002
Publication title -
journal of heredity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 92
eISSN - 1471-8505
pISSN - 0022-1503
DOI - 10.1093/jhered/93.4.279
Subject(s) - biology , oligochaeta (plant) , ribosomal dna , earthworm , lumbricidae , ribosomal rna , 18s ribosomal rna , genetics , 5s ribosomal rna , chromosome , gene , zoology , ecology , phylogenetics
Spermatogonial and metaphase I chromosomes of the lumbricid earthworm Octodrilus complanatus (Annelida: Oligochaeta) were examined using fluorescent in situ hybridization (FISH) with three repetitive DNA probes-5S rDNA, 18S-26S rDNA, and (TTAGGG)(n). Single-color FISH consistently mapped one chromosome pair per spread using either 5S rDNA or 18S-26S rDNA as probes. Simultaneous (18S-26S)-5S and (18S-26S)-(TTAGGG)(n) FISH demonstrated that repeated units of the two ribosomal families were overlapped and closely associated with telomeric sequences.
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