Brief communication. Heteroplasmy of mitochondrial DNA in the iphiuroid Astrobrachion constrictum | Zendy

D. J. Steel | Zendy

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Brief communication. Heteroplasmy of mitochondrial DNA in the iphiuroid Astrobrachion constrictum

Author(s) -

D. J. Steel

Publication year - 2000

Publication title -

journal of heredity

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.99

H-Index - 92

eISSN - 1471-8505

pISSN - 0022-1503

DOI - 10.1093/jhered/91.2.146

Subject(s) - heteroplasmy , biology , mitochondrial dna , genetics , single strand conformation polymorphism , haplotype , pseudogene , dna sequencing , gene , polymerase chain reaction , evolutionary biology , genome , genotype

We demonstrate the presence of mitochondrial heteroplasmy for the cytochrome oxidase I (COI) gene of the brittle star (Astrobrachion constrictum). One of the 117 individuals analyzed contained two distinct single-strand conformation polymorphism (SSCP) haplotypes differing by two substitutions; another showed sequence evidence for heteroplasmy. We used polymerase chain reaction (PCR) cloning, SSCP, and sequencing of a 480 bp region of the 5' end of COI to isolate and characterize these haplotypes. This is the first properly substantiated case of heteroplasmy in an echinoderm species and may have arisen from paternal leakage.

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