Brief communication. A cosmid specific for sequences encoding a microtubule-associated protein, MAP1B, contains a polymorphic microsatellite and maps to bovine chromosome 20q14 | Zendy

A. Eggen | Zendy; Sabina SolinasToldo | Zendy; Ruedi Fries | Zendy

Open Access

Brief communication. A cosmid specific for sequences encoding a microtubule-associated protein, MAP1B, contains a polymorphic microsatellite and maps to bovine chromosome 20q14

Author(s) -

A. Eggen,

Sabina SolinasToldo,

Ruedi Fries

Publication year - 1998

Publication title -

journal of heredity

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.99

H-Index - 92

eISSN - 1471-8505

pISSN - 0022-1503

DOI - 10.1093/jhered/89.4.359

Subject(s) - cosmid , biology , genetics , microbiology and biotechnology , microsatellite , genomic library , bovine genome , gene , genomic dna , gene mapping , clone (java method) , chromosome , allele , genome , peptide sequence

A clone for the bovine gene MAP1B was isolated from a bovine cosmid library with a probe obtained by cross-species PCR. The single positive cosmid clone was localized by FISH to chromosome region 20q14. Analysis of the cosmid revealed the presence of a microsatellite motif with a two-allele polymorphism detectable by PCR amplification of genomic DNA. However, the analysis of this polymorphism in a bovine family affected by SMA indicated absence of very close linkage of the phenotype for bovine SMA with MAP1B.

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