Intracellular accumulation of indium ions released from nanoparticles induces oxidative stress, proinflammatory response and DNA damage | Zendy

Yosuke Tabei | Zendy; Takaaki Sonoda | Zendy; Yoshihiro Nakajima | Zendy; Vasudevanpillai Biju | Zendy; Yoji Makita | Zendy; Yasukazu Yoshida | Zendy; Masanori Horie | Zendy

Open Access

Intracellular accumulation of indium ions released from nanoparticles induces oxidative stress, proinflammatory response and DNA damage

Author(s) -

Yosuke Tabei,

Takaaki Sonoda,

Yoshihiro Nakajima,

Vasudevanpillai Biju,

Yoji Makita,

Yasukazu Yoshida,

Masanori Horie

Publication year - 2015

Publication title -

the journal of biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.28

H-Index - 115

eISSN - 1756-2651

pISSN - 0021-924X

DOI - 10.1093/jb/mvv098

Subject(s) - indium tin oxide , intracellular , proinflammatory cytokine , indium , chemistry , oxidative stress , reactive oxygen species , dna damage , biophysics , comet assay , biochemistry , inflammation , biology , dna , immunology , organic chemistry , layer (electronics)

Due to the widespread use of indium tin oxide (ITO), it is important to investigate its effect on human health. In this study, we evaluated the cellular effects of ITO nanoparticles (NPs), indium chloride (InCl3) and tin chloride (SnCl3) using human lung epithelial A549 cells. Transmission electron microscopy and inductively coupled plasma mass spectrometry were employed to study cellular ITO NP uptake. Interestingly, greater uptake of ITO NPs was observed, as compared with soluble salts. ITO NP species released could be divided into two types: 'indium release ITO' or 'tin release ITO'. We incubated A549 cells with indium release ITO, tin release ITO, InCl3 or SnCl2 and investigated oxidative stress, proinflammatory response, cytotoxicity and DNA damage. We found that intracellular reactive oxygen species were increased in cells incubated with indium release ITO, but not tin release ITO, InCl3 or SnCl2. Messenger RNA and protein levels of the inflammatory marker, interleukin-8, also increased following exposure to indium release ITO. Furthermore, the alkaline comet assay revealed that intracellular accumulation of indium ions induced DNA damage. Our results demonstrate that the accumulation of ionic indium, but not ionic tin, from ITO NPs in the intracellular matrix has extensive cellular effects.

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