Lectin-dependent inhibition of antigen-antibody reaction: application for measuring 2,6-sialylated glycoforms of transferrin | Zendy

Koki Hoshi | Zendy; Yoshinobu Kariya | Zendy; Kiyomitsu Nara | Zendy; Hiromi Ito | Zendy; K. Matsumoto | Zendy; Masamichi Nagae | Zendy; Yoshiki Yamaguchi | Zendy; Madoka Nakajima | Zendy; M. Miyajima | Zendy; Hiroyuki Arai | Zendy; Atsushi Kuno | Zendy; Hisashi Narimatsu | Zendy; Keiro Shirotani | Zendy; Yasuhiro Hashimoto | Zendy

Open Access

Lectin-dependent inhibition of antigen-antibody reaction: application for measuring 2,6-sialylated glycoforms of transferrin

Author(s) -

Koki Hoshi,

Yoshinobu Kariya,

Kiyomitsu Nara,

Hiromi Ito,

K. Matsumoto,

Masamichi Nagae,

Yoshiki Yamaguchi,

Madoka Nakajima,

M. Miyajima,

Hiroyuki Arai,

Atsushi Kuno,

Hisashi Narimatsu,

Keiro Shirotani,

Yasuhiro Hashimoto

Publication year - 2013

Publication title -

the journal of biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.28

H-Index - 115

eISSN - 1756-2651

pISSN - 0021-924X

DOI - 10.1093/jb/mvt065

Subject(s) - glycobiology , library science , government (linguistics) , medicine , political science , chemistry , biochemistry , glycan , computer science , linguistics , philosophy , glycoprotein

We developed a high-throughput Enzyme-linked immunosorbent assay (ELISA) for measuring α2,6-sialylated transferrin (Tf), based on inhibition of anti-Tf antibody binding to α2,6-sialylated Tf by a lectin, Sambucus sieboldiana Agglutinin (SSA). The inhibition was not observed with other glycoforms, such as periodate-treated, sialidase-treated and sialidase/galactosidase-treated Tf, suggesting that the assay was glycoform specific. This finding was applied to an automated latex-agglutination immunoassay, using SSA lectin as an inhibitor (SSA-ALI). The concentration of α2,6-sialylated Tf measured by SSA-ALI in human cerebrospinal fluid was correlated with that of ELISA (r2 = 0.8554), previously developed for measuring α2,6-sialylated Tf.

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