Open AccessDetermination of Nine Environmental Phenols in Urine by Ultra-High-Performance Liquid Chromatography–Tandem Mass Spectrometry
Author(s) -
Minjian Chen,
Pengfei Zhu,
Bin Xu,
Risheng Zhao,
Shubin Qiao,
Xiaojiao Chen,
Rong Tang,
Di Wu,
Song Lee,
Shoulin Wang,
Yankai Xia,
Xinru Wang
Publication year - 2012
Publication title -
journal of analytical toxicology.
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.161
H-Index - 76
eISSN - 1945-2403
pISSN - 0146-4760
DOI - 10.1093/jat/bks072
Subject(s) - chemistry , chromatography , phenols , pentachlorophenol , triclosan , solid phase extraction , tandem mass spectrometry , extraction (chemistry) , mass spectrometry , liquid chromatography–mass spectrometry , high performance liquid chromatography , selected reaction monitoring , environmental chemistry , organic chemistry , medicine , pathology
A method was developed to determine nine environmental phenols, including bisphenol A, 2,3,4-trichlorophenol, 2,4,5-trichlorophenol, pentachlorophenol, triclosan (2,4,4'-trichloro-2'-hydroxyphenylether), 4-tert-octylphenol, 4-n-octylphenol, 4-n-nolyphenol and benzophenone-3 (2-hydroxy-4-methoxybenzophenone) in human urine using ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS-MS). The analytes were extracted and preconcentrated with solid-phase extraction, and then quantified with UPLC-electrospray ionization (negative ion mode)-MS-MS using multiple reaction monitoring mode. Limits of detection of the nine phenols ranged from 0.02 to 0.90 ng/mL. This method was further validated by the determination of phenols in 325 human urine samples that generated data regarding the exposure of various phenols to Chinese adults without occupational exposure to phenols.