Optimization of an LC-MS Method for the Determination of Artesunate and Dihydroartemisinin Plasma Levels using Liquid-Liquid Extraction | Zendy

S. A. A. Van Quekelberghe | Zendy; Shakeel Ahmed Soomro | Zendy; Jan Cordonnier | Zendy; Frans Herwig Jansen | Zendy

Open Access

Optimization of an LC-MS Method for the Determination of Artesunate and Dihydroartemisinin Plasma Levels using Liquid-Liquid Extraction

Author(s) -

S. A. A. Van Quekelberghe,

Shakeel Ahmed Soomro,

Jan Cordonnier,

Frans Herwig Jansen

Publication year - 2008

Publication title -

journal of analytical toxicology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.161

H-Index - 76

eISSN - 1945-2403

pISSN - 0146-4760

DOI - 10.1093/jat/32.2.133

Subject(s) - artesunate , dihydroartemisinin , chromatography , chemistry , artemisinin , formic acid , mass spectrometry , atmospheric pressure chemical ionization , liquid chromatography–mass spectrometry , selected reaction monitoring , artemether , bioanalysis , ethyl acetate , extraction (chemistry) , chemical ionization , tandem mass spectrometry , plasmodium falciparum , ionization , organic chemistry , malaria , immunology , ion , biology

Artesunate is a derivate of artemisinin, an antimalarial drug used for the treatment of malaria caused by Plasmodium falciparum and related parasites. Artesunate is hydrolyzed rapidly to dihydroartemisinin in vivo. It has been found that artemisinin and its derivatives may have neurotoxic effects. A method was developed to analyze human plasma samples for the contents of artesunate and dihydroartemisinin. The plasma samples are extracted with ethyl acetate, concentrated, and redissolved in water/acetonitrile. Analyses was performed with liquid chromatography-mass spectrometry using a binary gradient program with aquaeous formic acid and acetonitrile formic acid on a XTerra MS C18-column. The mass spectrometer was operated in the positive atmospheric pressure chemical ionization mode with single ion recording. The lower limits of detection were 10 and 25 ng/mL plasma for DHA and artesunate, respectively. The method was validated according to the guidelines for validation of bioanalytical methods.

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