Open AccessDetection of Recombinant Epoetin and Darbepoetin Alpha after Subcutaneous Administration in the Horse
Author(s) -
Françoise Lasne,
MarieAgnès Popot,
Emmanuelle VarletMarie,
Laurent Martin,
JeanAntoine Martin,
Yves Bonnaire,
Michel Audran,
J. de Céaurriz
Publication year - 2005
Publication title -
journal of analytical toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.161
H-Index - 76
eISSN - 1945-2403
pISSN - 0146-4760
DOI - 10.1093/jat/29.8.835
Subject(s) - erythropoietin , darbepoetin alfa , recombinant dna , horse , urine , isoelectric focusing , alpha (finance) , medicine , chemistry , pharmacology , endocrinology , chromatography , enzyme , biology , biochemistry , surgery , paleontology , construct validity , patient satisfaction , gene
A direct detection method for anti-doping control of recombinant human erythropoietin (rHuEPO) abuse in racehorses is proposed. This method involves screening of plasma (or serum) by an enzyme-linked immunosorbent assay specific for human EPO and confirmation in urine samples by characterization of the urinary EPO isoelectric profile. This method was tested on horses that were administered epoetin alpha (rHuEPO) and the hyper-glycosylated form of this drug (darbepoetin alpha).
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