Investigation of an LC-MS-MS (QTrap(R)) Method for the Rapid Screening and Identification of Drugs in Postmortem Toxicology Whole Blood Samples | Zendy

G Herrin | Zendy; H. Horton McCurdy | Zendy; William H. Wall | Zendy

Open Access

Investigation of an LC-MS-MS (QTrap(R)) Method for the Rapid Screening and Identification of Drugs in Postmortem Toxicology Whole Blood Samples

Author(s) -

G Herrin,

H. Horton McCurdy,

William H. Wall

Publication year - 2005

Publication title -

journal of analytical toxicology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.161

H-Index - 76

eISSN - 1945-2403

pISSN - 0146-4760

DOI - 10.1093/jat/29.7.599

Subject(s) - forensic toxicology , clinical toxicology , chromatography , chemistry , pharmacology , medicine , toxicology , biology

The QTrap liquid chromatography-tandem mass spectrometry (LC-MS-MS) by Applied Biosystems was investigated as an adjund to enzyme immunoassay (EIA) for the rapid detection of drugs in blood. Thus, a procedure used to identify drugs in whole blood by EIA was extended to LC-MS-MS analysis. A multiple reaction monitoring (MRM) database of over 100 drugs was constructed to analyze for those drugs commonly observed in postmortem toxicology cases. The QTrap method provided for a scan time of only 2.8 s to produce both an MRM and an enhanced product ion scan. Various validation and developmental steps of the method are presented, as well as a concordance study as a final means of validation. This study was conducted to compare the effectiveness of the QTrap versus conventional extraction methods and gas chromatography-MS for the identification of drugs in 95 postmortem samples. The more than 400 drug results in this study showed 100% concordance between the two techniques.

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