Isotope Dilution-Mass Spectrometry Determination of Blood Cyanide by Headspace Gas Chromatography | Zendy

Pierre Dumas | Zendy; Guy Gingras | Zendy; Alain LeBlanc | Zendy

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Isotope Dilution-Mass Spectrometry Determination of Blood Cyanide by Headspace Gas Chromatography

Author(s) -

Pierre Dumas,

Guy Gingras,

Alain LeBlanc

Publication year - 2005

Publication title -

journal of analytical toxicology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.161

H-Index - 76

eISSN - 1945-2403

pISSN - 0146-4760

DOI - 10.1093/jat/29.1.71

Subject(s) - isotope dilution , chemistry , chromatography , mass spectrometry , cyanide , phosphoric acid , dilution , detection limit , gas chromatography–mass spectrometry , gas chromatography , analytical chemistry (journal) , inorganic chemistry , organic chemistry , physics , thermodynamics

A direct and sensitive method for the determination of blood cyanide by isotope dilution was developed. The blood is placed in a headspace vial, and K13C15N is added as internal standard. Addition of phosphoric acid liberates the cyanide as HCN. The detection is accomplished by mass spectrometry after a fine mass calibration tuning. The detection limit obtained is 0.3 micromol/L. The within- and inter-run coefficients of variation are 4.4% (for a concentration of 2.5 micromol/L) and 3.9% (for a concentration of 4.7 micromol/L), respectively. The observed recovery is 98%. A round-robin exercise was carried out to compare the performance of this method with others currently in use in other clinical laboratories.

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