A Rapid Procedure for the Monitoring of Amiodarone and N-Desethylamiodarone by HPLC-UV Detection | Zendy

JoEtta M. Juenke | Zendy; Paul I. Brown | Zendy; Gwendolyn A. McMillin | Zendy; Francis M. Urry | Zendy

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A Rapid Procedure for the Monitoring of Amiodarone and N-Desethylamiodarone by HPLC-UV Detection

Author(s) -

JoEtta M. Juenke,

Paul I. Brown,

Gwendolyn A. McMillin,

Francis M. Urry

Publication year - 2004

Publication title -

journal of analytical toxicology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.161

H-Index - 76

eISSN - 1945-2403

pISSN - 0146-4760

DOI - 10.1093/jat/28.1.63

Subject(s) - chromatography , high performance liquid chromatography , metabolite , amiodarone , detection limit , therapeutic drug monitoring , chemistry , extraction (chemistry) , quantitative analysis (chemistry) , active metabolite , drug , pharmacology , medicine , biochemistry , atrial fibrillation , cardiology

This article describes a rapid isocratic high-performance liquid chromatographic (HPLC) method for the simultaneous measurement of the antiarrhythmic drug amiodarone and its potentially active metabolite N-desethylamiodarone (DEA). Following a simple liquid-liquid extraction, amiodarone and its metabolite are quantitated (0.3-6.0 mg/L) by analysis on an HPLC-UV system. The analytical time was reduced by 50%, without compromising the assay performance, when Rocket column technology was employed. The assay's limit of quantitation, linearity, imprecision, and accuracy adequately covered the therapeutic range for appropriate patient monitoring. Amiodarone and DEA can be simultaneously and accurately quantitated in serum or plasma by HPLC-UV detection with imprecision < 6% at therapeutic concentrations and a quantitation range from 0.3 to 6.0 mg/L. Monitoring of this drug can allow for effective use, while minimizing serious side effects.

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