Screening Method for Seventy Psychoactive Drugs or Drug Metabolites in Serum Based on High-Performance Liquid Chromatography-Electrospray Ionization Mass Spectrometry | Zendy

Martin Rittner | Zendy; Fritz Pragst | Zendy; Wolf -Rainer Bork | Zendy; Joachim Neumann | Zendy

Open Access

Screening Method for Seventy Psychoactive Drugs or Drug Metabolites in Serum Based on High-Performance Liquid Chromatography-Electrospray Ionization Mass Spectrometry

Author(s) -

Martin Rittner,

Fritz Pragst,

Wolf -Rainer Bork,

Joachim Neumann

Publication year - 2001

Publication title -

journal of analytical toxicology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.161

H-Index - 76

eISSN - 1945-2403

pISSN - 0146-4760

DOI - 10.1093/jat/25.2.115

Subject(s) - chromatography , mass spectrometry , electrospray ionization , chemistry , electrospray , solid phase extraction , mass spectrum

A screening method for 70 psychoactive drugs or drug metabolites in human serum by solid-phase extraction with subsequent high-performance liquid chromatography and electrospray ionization mass spectrometry was developed. Enhanced selectivity of detection was obtained by collision-induced dissociation using two different skimmer voltages for the individual scan. The mass spectra and the retention times of the compounds were incorporated into a self-generated spectra library for identification in screening experiments. The detection limits were found to be between 0.1 and 5 ng/mL serum for the majority of the compounds when measured in the selected ion mode. Because of the very small serum concentrations and the rather low extraction yield, lysergide could not be detected in this way. It was demonstrated with 140 serum samples from alcohol-related traffic cases that this method is suitable for a routine screening in a forensic laboratory.

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